Academisch Medisch Centrum (AMC) bij de Universiteit van Amsterdam (UVA) – NL

amcGeneral description:

The department of Endocrinology and Metabolism of the Academic Medical Center (AMC) has a longstanding expertise in studying the physiology and pathophysiology of glucose and lipid metabolism in humans with a special focus on translational research.

Main areas of interest are human models of insulin resistance and the role of the brain in the regulation of glucose and lipid metabolism. The SERLIE group works in close collaboration with the departments of medical biochemistry, experimental endocrinology, nuclear medicine, radiology and vascular medicine besides national and international collaborations. In 2011, the department started a consortium with several surgical centers in the Netherlands to establish a biobank containing human tissue samples (liver, adipose tissue) and blood of patients undergoing bariatric surgery. Recently, the department started collaboration with a systems biology group to model glucose tolerance using an oral glucose load. The department of Vascular medicine (chairs Prof dr E.S.G. Stroes, prof dr H.R. Buller and prof dr J.J.P. Kastelein) is at the forefront of translational vascular medicine and lipidology. Researchline included novel pathophysiological pathways involved in atherosclerosis (inflammation by innate immunity and gutmicrobiota) and dyslipidemia in metabolic syndrome and type 2 diabetes mellitus as well as novel therapeutic interventions aimed against cardiovascular events (antisense strategies for cholesterol lowering, CETP inhibitors).

Website:

http://www.amc.nl/web/Research.htm

Expertise:

Dept of Endocrinology and Metabolism: performing studies in human models of insulin resistance; combining measurements of metabolic fluxes with tissue biopsies to study metabolic pathways involved in glucose and lipid metabolism. Dept. of Vascular medicine ample experience in studying lipidfluxes in human subjects by using stable isotopes including intestinal uptake of cholesterol (see Nierman, ATVB 2006), apoA-1 fluxes (see Franssen, ATvB 2009) and lipolyses fluxes of TG-VLDL synthesis (using 1,1,3,3, H5 glycerol, see Sondermeijer, submitted).

Facilities:

fully equipped metabolic research unit, fully equipped to measure the relevant isotope tracers. Extended oral glucose tolerance tests, mixed meal tests as well as hyperinsulinemic euglycemic clamps are performed and combined with muscle and adipose tissue biopsies. Indirect calorimetry is used to measure resting energy expenditure.